Abstract

The Rhizosphere is a hotspot of plant-microbe interactions with profound influence on plant productivity and ecosystem function. The aim of this research is to isolate and identify Rhizosphere fungi from Poaceae and Cyperaceae family grass for agricultural application to use as biofertilizer, bio control and bio pesticide. Rhizosphere soil and root sample were collected from 18 Poaceae and Cyperaceae family grass. Malt and potato dextrose agar were used to primary and secondary fungi culture. Pure fungi isolates were transferred to biolog universal yeast agar media. Pure yeast cells and filamentous fungi were suspended in sterile water and filamentous fungi (FF) inoculums fluid at 49±2 and 75±2 turbidity measured by biolog turbidimeter, respectively. 100 μL transferred from each suspension into 96 wells of the biolog yeast micro plate and filamentous fungi micro plate tagged with different carbon source and incubated at 26°C for 24 to 72 h for yeast and 24-240 h for filamentous fungi and read by micro station at a single wavelength of 590 nm, The biolog software micro log3 ver. 4.20.05 compared the results obtained with the test strain to the database and provided identification based on distance value of match and separation score produces similarity index value and probability results were recorded and processed for identification by micro log³ software ver. 4.20.05. Biolog microstation read 27 fungi species. The result revealed that Fellomyces fuzhouensis, Schizoblastosporion starkeyi-henricii, Cryptococcus luteolus, Rhodotorula aurantiaca A, Cryptococcus Terries A, Trichosporon beigelii B, Rhodotorula aurantiaca B, Cryptococcus albidus var aerius, were fully identified above 75% probability and 0.5 similarity index. In conclusion these rhizosphere- microbes interaction study useful for agricultural application for bio fertilizer after further evaluation.