Abstract

Analytical chemistry uses high-performance liquid Chromatography (HPLC), originally known as high pressure liquid Chromatography, to separate, identify, and quantify components in mixtures. The mixtures may come from liquid solutions containing food, chemicals, medicines biological, environmental, and agricultural materials, among other sources. In addition to a range of other human and animal studies, the development and validation of HPLC techniques play crucial roles in the discovery, development, and production of innovative drugs. This article covers the use of HPLC to separate the medication Irbesartan. Evaluating the quantity of drug is one way to perform drug quality assurance, which is necessary to ensure the safety and quality of pharmaceutical products. In this work, a stability indicating RP-HPLC technique for estimating Irbesartan degradation and process-related contaminants is developed and validated. An addition to pharmaceutical preparations it can also be used for biological matrices samples that require a small amount of sample analysis. The assay and impurity levels in bulk active and prepared medicinal products are frequently determined using high performance liquid Chromatography (HPLC). To demonstrate the capacity to identify a broad range of impurities that may arise in pharmaceuticals, impurity profile analyses are necessary. HPLC instrumentation consists of a solvent reservoir, pump, injector, column, detector, integrator, or acquisition and display system. The brain of the system is the column where separation occurs. Among other things, HPLC can be used to identify, quantify, and resolve a compound. For the purpose of quality control of medication components, it is the most adaptable, secure, trustworthy, and rapid chromatographic technology available.