Plant growth promoting bacteria used as biofertilizers for nutrient solubilization and nitrogen fixation also has the potential to suppress disease causing pathogens. This study aims to investigate the antagonistic action of plant growth promoting Pseudomonas (P), Azotobacter (Azb) and Azospirillum (Asp) to suppress the growth of agriculturally important five fungal phytopathogens viz. Macrophomina phaseolina, Sclerotium rolfsii, Rhizoctonia solani, Fusarium oxysporum f.sp. ricini and Alternaria tenuissima. Out of 50 strains of Pseudomonas, 38 strains of Azotobacter and 41 strains of Azospirillum tested seven strains of Pseudomonas (P88, P95, P101, P105, P107, P115, P124) Five strains of Azotobacter (Azb 2, 6, 10, 16, 18) and six strains of Azospirillum (Asp2, 10, 22, 30, 32 and 39) showed the inhibition of mycelium development of all the five major soil borne phytopathogens. Therefore, invitro screening for antagonistic ability provided the basis for identification and selection of plant growth promoting strains with potent biocontrol ability.