Abstract

Tomato spotted wilt virus (TSWV), which is a member of the Orthotospovirus genus and transmitted by thrips. It has extensive host and causes significantly economic losses. In May 2023, virus-like symptoms such as ring spotted, leaf yellowing and necrosis were observed on Agapanthus praecox leaves in Kunming, Yunnan Province, China. Spherical viral particles with a diameter of 80-120nm were observed by TEM. The results of RT-PCR and DNA showed that the sequence of N gene for this virus shared 99.87% identity with TSWV isolate from Shandong isolate (acc. no. OQ627140.1). The obtained 777-bp consensus sequence named TSWN-N was deposited in GenBank (acc. no. OP867047.1). Phylogenetic tree analyses showed that TSWV obtained from Agapanthus praecox plant samples clustered together into a large clade from Guizhou. Koch’s postulate and the virus was reamplied from the inoculated plants. This is the first report of TSWV on Agapanthus praecoxin China and provides early warning of virus disease on medicinal materia.