Abstract

Horticultural crops comprise a wide variety of fruits, vegetables, nuts, flowers, fragrant and medicinal plants. They provide nutritional, medical, and aesthetic advantages to humans. However, these crops face several biotic and abiotic stress. Thus, now a days conventional breeding and biotechnology methods are being used to generate improved cultivars. Recent novel plant breeding techniques, such as the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated 9 (CRISPR/ Cas9) system, have gained popularity owing to their efficiency, accuracy and simplicity when compared to older genome editing methods. Increased understanding of the efficacy and efficiency of biotechnology techniques may inform the usage of CRISPR gene-editing technology. In this review article, we look at CRISPR/Cas9 technology, including its history, categorization, general applications, particular usage in horticulture crops, obstacles, current resources, regulatory implications, and the way ahead. We also highlighted the use of CRISPR technology in horticulture plants to increase stress responses, fruit quality, and cultivation features. This complete analysis was created to assist construct a full knowledge of the CRISPR/Cas9 systems and to serve as a reference for future development of this technology to efficiently control horticultural plant features.