To study the knowledge and attitude among healthcare workers regarding bio-medical waste management at SKIMS, a crosssectional study in prospective design were conducted in randomly selected areas.The study population included doctors, nurses, technician and sanitary staff. A semi-structured questionnaire was adapted from different research papers were used as a study tool. Observation revealed that among the entire study group doctors had a highest mean knowledge score, followed by nursing staff.Lowest mean knowledge score was found in sanitary attendants. There was a significant difference is mean knowledge score of postgraduate nurses who had a higher mean knowledge score compared to undergraduates having a mean knowledge score.