Abstract

We aimed to establish the chemical constituents of essential oil and biological assays from crude ethanolic extract of ginger from Sindhupalchhowk, Tanahu and Gorkha region of Nepal. Ginger essential oils were extracted using hydro distillation, and having the yield percent; Gorkha: 1.9, Sindhupalchowk: 1.8 and Tanahu: 1.1. GC-MS analysis revealed the presence of 105, 85, 88 components from Sindhupalchowk, Tanahu and Gorkha ginger respectively, with the major constitutes, monoterpenes and sesquiterpenes derivatives. The Soxhlet extraction was performed for the 95 % ethanolic extraction from fresh ginger (EE) and the residue ginger after essential oil extraction (EEROE). The antimicrobial activity of 8% EE & EEROE against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Klebsiella spp. was expressed in zone of inhibition (mm) and comparable with chloramphenicol Similarly, the antioxidant assay was performed by using DPPH radical scavenging method in different concentration. Where, EE & EEROE showed the maximum percentage inhibition as compared with ascorbic acid but the essential oil did not possess the antioxidant activity.