Abstract

Phytoconstituents, minerals, and vitamins have been widely evaluated for their significant biological properties. A new proprietary test formulation was prepared with a mixture of the nanocurcumin, zinc chloride, magnesium (II) gluconate, sodium selenate, ascorbic acid (vitamin C), cholecalciferol (vitamin D3), iron (II) sulfate, and copper chloride. The study was aimed to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) Treatment on the test formulation in splenocyte cells isolated from the Biofield Energy Treated mice. The test formulation was distributed in two different parts, one part was denoted as control and the other part was demarcated as the Biofield Energy Treated sample, which received Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mahendra Kumar Trivedi. MTT assay data showed more than 70% cell viability in the test formulation group and was found to be safe and non-cytotoxic in splenocyte cells. Proinflammatory cytokine tumor necrosis factor-α (TNF-α) expression was significantly (p≤0.01) reduced by 25.73%, 19.05%, and 18.05% at 0.1, 0.5, and 5.2 µg/mL, respectively as compared to the vehicle control (VC) group. The level of macrophage inflammatory proteins-1α (MIP-1α) was significantly reduced by 20.32% in the Biofield Energy Treated test formulation at 3 µg/mL than VC group. Additionally, the level of interleukin-1β (IL-1β) secretion was significantly inhibited in the Biofield Energy Treated test formulation by 16.09% and 10.71% at 0.1 and 1 µg/mL, respectively with respect to the VC group. Overall, results suggest that The Trivedi Effect®-Consciousness Energy Healing Treatment has significantly improved and modulate the immunomodulatory effect of the test formulation in the Biofield Treated mouse splenocytes. Therefore, data anticipated that the Biofield Energy Treated test formulation can be utilized for the management of many inflammatory disorders viz. rheumatoid arthritis, inflammatory bowel diseases, psoriasis, scleroderma, multiple sclerosis, and type 1 diabetes mellitus, aging, stress, and immune-related disease conditions.