Abstract

The study objective was to evaluate the immunomodulatory activity of The Trivedi Effect®-Consciousness Energy Healing based new proprietary test formulation and the Biofield Energy Treatment per se on male rats. The test formulation was divided into two parts, one part was denoted as the untreated formulation, while the other was treated with the Biofield Energy by Mahendra Kumar Trivedi and denoted as the Biofield Energy Treated test formulation. Besides, three group of animals were also received Biofield Energy Treatment under same conditions. Immunomodulatory action was evaluated using estimation of CSF biomarkers, cellular immune response, immunoglobulin identification, hematology, biochemistry, and analysis of testosterone level. The experimental groups included normal control (G1), disease control (G2), positive control; levamisole hydrochloride (G3), untreated test formulation (G4), Biofield Energy Treated test formulation (G5), Biofield Energy Treatment per se to animals at day -15 (G6), Biofield Energy Treated test formulation from day -15 (G7), Biofield Energy Treatment per se to the animals along with Biofield Treated test formulation from day -15 (G8), and Biofield treatment per se to animals with untreated test formulation (G9). CSF biomarkers such as serotonin and dopamine levels were significantly increased upto 85.46% (G8) and 97.84% (G8), respectively; while corticosterone level was decreased by 58.75% (G7) as compared to the G2. Biofield Treatment per se improved the level of immunoglobulin E (IgE) by 28.82% in G6 than G2. Biofield Energy Treatment significantly increased the percentage of cellular biomarkers like CD4+, CD8+, and CD28+ by 24.45%, 22.78%, and 28.37%, respectively in the G5; while increased by 29.43%, 25%, and 30.82%, respectively in the G9 as compared to the G2. Neutrophils and total leukocyte count (TLC) were significantly increased by 59.52% and 16.66%, respectively in G5 group compared with the G2 group. The percentage of triglycerides was significantly decreased by 22.39% and 16.46% in G5 and G7 groups, respectively, while very low density lipoprotein (VLDL) level was significantly decreased by 22.84% and 16.50% in G5 and G7 groups, respectively as compared with the G2. Hepatic biomarkers, serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvate transaminase (SGPT) were decreased by 7.61% and 14.10%, respectively in G5 group. However, level of creatine kinase myocardial band (CK-MB) was reduced by 11.34% and 20.55% in G5 and G9 groups, respectively as compared with G2 group. However, significant increased testosterone level was found by 28.71% in G7 as compared with the G2 group. Overall, data suggest that Biofield Energy Treatment per se (The Trivedi Effect®) and Biofield Treated test formulation have significant immunomodulatory effect, as compared with the untreated formulation in order to improve and boost the immune system. Therefore, this therapy could be useful for the management of stress and various immune-related disorders like Rheumatoid Arthritis, Myasthenia Gravis, Systemic Lupus Erythematosus, Aplastic Anemia, Addison Disease, Pernicious Anemia, Reactive Arthritis, Graves’ Disease, Multiple Sclerosis, etc.