Abstract

Hyaluronan (HA), a component of the extracellular matrix, has been implicated in regulating angiogenesis and cell proliferation, migration, and signaling. We used the rat MCAO to show hyaluronan accumulation in stroke-affected areas. Using RT-PCR and Western blotting we showed up-regulation of hyaluronidase-1 and 2 between 1h and 21 days after stroke. Hyaluronidase-1 was up regulated earlier than hyaluronidase-2. RHAMM and CD44 receptors were also increasedafter stroke. Immunohistochemistry results, showed an association of hyaluronidases1/2 and hyaladherins with neurons in the infarcted and peri-infarcted regions and hyaluronidase-1 with micro vessels. HA synthesis and degradation in the stroke hemisphere might have an impact on neuronal survival, angiogenesis and general tissue remodeling after stroke.