Evidence collection in forensic investigation contribute very important role. There are different types of evidence among which saliva plays an important role. For forensic purpose. DNA can be isolated from saliva samples whereas collection requires high precaution and precision training. Salivary samples can be collected from food remaining or even from cigarette butts, also from body parts in crime scenes. Salivary microorganisms differ from one individual to other thereby reflecting their health status and dietary habits. Hence Salivary microbiome plays an important role in forensic investigation. With the help of these salivary microbial analysis; individual age, ethnicity, and so on information can be used for forensic contexts. 16S rRNA gene sequencing is used for forensic microbiome analyses. This mini review comprises the advantages and some insights of salivary microbiome in forensic investigations.