Screening for contaminants in food and feed is of vital importance. A method has been developed for Zearalenone (Zen) testing by combined enzyme-linked immunosorbent assay (ELISA) and column immunoaffinity chromatography. Zen is a highly toxic mycotoxin present in food and feed. The authors generated a monoclonal antibody (mAb) with high specificity for Zen. The mAbs developed had no cross-reactivity with other mycotoxins. The mAbs did recognize related Zen structures. Column immunoaffinity chromatography was then developed using the mAbs. The dual testing format was used to test for Zen artificially treated, in a dose dependent manner, food and feed samples. The successful results allowed the authors to then use their testing strategy on food and feed samples from a local market. Again positive results were obtained.