Introduction: The Aseptic Non-Touch Technique (ANTT) is a fundamental medical and nursing skill that describes the infection prevention and control methodologies and precautions required during invasive clinical procedures to reduce the risk of transmission of microorganisms from health care providers, equipment, or the environment to a patient. It is essential that healthcare providers have a thorough understanding of the dangers they pose to patients and that this knowledge can be demonstrated in practice. This review protocol aims to provide an in-depth analysis of the current state of healthcare care provider knowledge, attitude, and practice of ANTT.

Methods: This study will be conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA)-2009 with the use of sample, Phenomenon of Interest, Design, Evaluation, and Research Type (SPIDER) and Population, Intervention, Comparison, and Outcome (PICO) tools to structure the research question. The following databases will be searched to identify eligible published papers (CINAHL, PUBMED/Medline, Scopus, Web of Science, Embase, Cochrane Library) and unpublished papers and ‘grey’ literature (ProQuest Dissertations & Theses, Open SIGLE and the Grey literature report).

Discussion: The review protocol will serve as a valuable resource for healthcare professionals, policymakers, educators, and researchers working towards enhancing patient safety and reducing HAIs.