Abstract

The ethanol extract of Polygonatum sibiricum leaves was investigated for its anti-osteoporosis effects in a glucocorticoidinduced osteoporosis rat model. Biochemical markers such as serum calcium, phosphate, magnesium, creatinine, and alkaline phosphatase were measured. At a dose of 400 mg/kg body weight, the extract reduced serum calcium and phosphate levels by 54.01% and 27.73%, respectively, while increasing serum magnesium levels by 20% compared to the glucocorticoid-treated group. Additionally, creatinine and alkaline phosphatase levels were significantly reduced by 29.41% and 27.83%, respectively. Biomechanical analysis further supported these findings, showing that the extract at the same dose enhanced bone strength and trabecular thickness without affecting bone length compared to the diseased group. The extract also improved the cortical and trabecular bone architecture, resulting in a well-organized bone matrix. These results suggest that Polygonatum sibiricum leaf extract holds potential therapeutic value for treating osteoporosis.